分泌型糖蛋白（SFPR2）的酶联免疫吸附试验试剂盒
| 产品描述： | SFRP2 是一类分泌型糖蛋白，通过与 Wnt 配体或 Frizzled 受体结合调控经典与非经典 Wnt 信号通路，在胚胎发育的组织极性建立、细胞增殖分化中起关键作用。其表达异常与肿瘤微环境重塑、器官纤维化、骨代谢失衡等病理过程密切相关，是解析 Wnt 信号通路异常激活机制的核心分子标志物，在肿瘤生物学与发育生物学研究中具有重要科研价值。 |
| 别名： | 分泌型卷曲相关蛋白 2、Frizzled 相关蛋白 2 |
| 缩写： | SFRP2 |
| Uniprot No： | O43501 |
| 种属： | Mus musculus (Mouse) |
| 样本类型： | serum, plasma, tissue homogenates, cell culture supernates |
| 检测范围： | 15.62-1000pg/mL |
| 反应时间： | 1-5h |
| 样本体积： | 50-100ul |
| 检测波长： | 450 nm |
| 测定原理： | quantitative |
| 测定方法： | Sandwich |
| 货期： | 3-5 working days |
| 本试剂盒所含 材料： | A micro ELISA plat 8×12 |
| Two vials lyophilized standarde | |
| Concentrated Enzyme-Labeled Detection Antibody (100 x concentrate) 1 x 120 μl | |
| Universal Diluent 2 x 20 ml | |
| Wash Buffer (20 x concentrate) 2 x 10 ml | |
| TMB Substrate 1 x 10 ml | |
| Stop Solution 1 x 60 ml | |
| Four plate sealing film (For 96 wells) | |
| An instruction manua | |
| 精密度： | ntra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | |
| Three samples of known concentration were tested in twenty assays to assess. |
| 线性度： | To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||
Sample | Serum(n=4) | ||
1:200 | Average % | 97 | |
Range % | 92-104 | ||
1:400 | Average % | 91 | |
Range % | 82-98 | ||
1:800 | Average % | 103 | |
Range % | 99-111 | ||
1:1600 | Average % | 87 | |
Range % | 82-96 | ||
| 回收率： | The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 90 | 85-98 | |
EDTA plasma (n=4) | 112 | 105-117 | |
| 标准曲线： | 这些标准曲线仅用于演示。应为每组分析的样品生成标准曲线。 | ||
| 靶点详情： | SFRP2 是分泌型卷曲相关蛋白家族成员，为含 314 个氨基酸的糖蛋白，分子结构包含 N 端富含半胱氨酸的 CRD 结构域（与 Frizzled 受体同源）和 C 端净电荷区域。其核心功能是作为 Wnt 信号通路的关键调节剂，通过 CRD 结构域竞争性结合 Wnt 配体或直接与 Frizzled 受体相互作用，抑制经典 Wnt/β- 连环蛋白通路的激活，同时可调控非经典 Wnt 通路活性，参与细胞命运决定与组织稳态维持。生理状态下，SFRP2 在胚胎发育中发挥重要作用：在肢体发育阶段，通过抑制 Wnt 信号维持指间间充质细胞凋亡，调控指节形成；在神经管闭合过程中，参与神经上皮细胞极性建立；成年期则主要表达于肾脏、肺脏等组织，调控干细胞增殖与分化平衡。 |
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