α-L - 岩藻糖苷酶 1（FUCA1）酶联免疫吸附试验试剂盒
| 产品描述： | 本试剂盒采用双抗夹心法 ELISA 技术，专为科研场景设计，可定量检测样本中 FUCA1 蛋白浓度，检测范围为 10-1000pg/mL，包含预包被特异性捕获抗体的微孔板、HRP 标记检测抗体及配套试剂，特异性强（与其他糖苷水解酶交叉反应率＜1%），适用于细胞裂解液、组织匀浆、溶酶体提取物等样本。FUCA1 作为 α-L - 岩藻糖苷酶家族核心成员，在糖蛋白 / 糖脂降解及细胞外基质重塑中起关键作用，其表达异常与溶酶体贮积症模型、肿瘤微环境代谢重编程相关；机制上，其催化结构域通过水解糖链末端岩藻糖残基，调控细胞表面糖基化修饰（如整合素 α5β1 岩藻糖基化），影响细胞黏附与信号传导，本试剂盒可捕捉其表达变化以解析糖代谢调控机制。分子特征体现为含保守催化结构域（GH29 家族特征序列）及潜在 N - 糖基化位点，结构域协同实现底物特异性识别与水解功能；作为 “糖基化修饰调节器”，参与胚胎发育糖脂代谢、肿瘤模型细胞侵袭及免疫细胞糖基化表型调控。支持溶酶体功能缺陷模型中与糖贮积的关联研究、肿瘤细胞外基质岩藻糖基化通路解析及化合物对其催化活性的干预评估，为糖生物学机制研究提供可靠工具。 |
| 别名： | α-L - 岩藻糖苷酶 1、溶酶体岩藻糖苷酶 |
| 缩写： | FUCA1 |
| Uniprot No： | P04066 |
| 种属： | Mus musculus (Mouse) |
| 样本类型： | serum, plasma, tissue homogenates, cell culture supernates |
| 检测范围： | 100-1000pg/mL |
| 反应时间： | 1-5h |
| 样本体积： | 50-100ul |
| 检测波长： | 450 nm |
| 测定原理： | quantitative |
| 测定方法： | Sandwich |
| 货期： | 3-5 working days |
| 本试剂盒所含 材料： | A micro ELISA plat 8×12 |
| Two vials lyophilized standarde | |
| Concentrated Enzyme-Labeled Detection Antibody (100 x concentrate) 1 x 120 μl | |
| Universal Diluent 2 x 20 ml | |
| Wash Buffer (20 x concentrate) 2 x 10 ml | |
| TMB Substrate 1 x 10 ml | |
| Stop Solution 1 x 60 ml | |
| Four plate sealing film (For 96 wells) | |
| An instruction manua | |
| 精密度： | ntra-assay Precision (Precision within an assay): CV%<8% |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | |
| Three samples of known concentration were tested in twenty assays to assess. |
| 线性度： | To assess the linearity of the assay, samples were spiked with high concentrations of human ACEⅠ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||
| Sample | Serum(n=4) | ||
| 1:200 | Average % | 97 | |
| Range % | 92-104 | ||
| 1:400 | Average % | 91 | |
| Range % | 82-98 | ||
| 1:800 | Average % | 103 | |
| Range % | 99-111 | ||
| 1:1600 | Average % | 87 | |
| Range % | 82-96 | ||
| 回收率： | The recovery of human ACEⅠ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 90 | 85-98 | |
| EDTA plasma (n=4) | 112 | 105-117 | |
| 标准曲线： | 这些标准曲线仅用于演示。应为每组分析的样品生成标准曲线。 | ||
| 靶点详情： | α-L - 岩藻糖苷酶 1（FUCA1）是糖苷水解酶 29 家族（GH29）成员，为溶酶体酸性水解酶，含信号肽（介导溶酶体靶向）、催化结构域（含 GH29 家族保守活性位点 Glu274/Glu351）及富含半胱氨酸的 C 端结构域，催化结构域的构象变化可调控底物特异性，是其实现岩藻糖苷键水解功能的分子基础。主要定位于溶酶体，少量分泌至细胞外。其核心功能是通过水解糖蛋白、糖脂及蛋白聚糖末端的 α-L - 岩藻糖苷键，参与糖复合物降解与回收，维持细胞内糖代谢稳态。在科研模型中，FUCA1 缺失可导致岩藻糖基化底物蓄积，引发溶酶体功能紊乱；其异常高表达则通过重塑肿瘤细胞表面糖基化谱，增强与细胞外基质的相互作用，促进侵袭转移. |
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